This showed oocytes denudation process. (A) Cumulus Oocytes Complex prepared for mechanical denudation without enzyme; (B) Cumulus Oocytes Complex enzymolysed in hyaluronidase; (C) “Naked” oocytes following enzymolysed in hyaluronidase – clean edge; (D) “Naked” oocytes following mechanical denudation – minimal cumulus cell at the edge

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